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 Publications 

2023

M.A. Bodescu, J. Aretz, M. Grison, M. Rief, and Reinhard Fässler

Kindlin stabilizes the talin-integrin bond under mechanical load by generating an ideal bond.

Proc Natl Acad Sci USA 2023; 120: e2218116120

https://www.pnas.org/doi/epdf/10.1073/pnas.2218116120

2022

Moessmer, P., Suren, T., Majdic, U., Dahiya, V., Rutz, D., Buchner, J., and Rief, M.

Active unfolding of the glucocorticoid receptor by the Hsp70/Hsp40 chaperone system in single-molecule mechanical experiments.

Proc Natl Acad Sci U S A 2022; 119: e2119076119

https://www.pnas.org/doi/10.1073/pnas.2119076119

Dahiya, V., Rutz, D.A., Moessmer, P., Muhlhofer, M., Lawatscheck, J., Rief, M., and Buchner, J.

The switch from client holding to folding in the Hsp70/Hsp90 chaperone machineries is regulated by a direct interplay between co-chaperones.

Mol Cell 2022; 82: 1543-1556 e1546

https://www.sciencedirect.com/science/article/abs/pii/S1097276522000594

Jones, A.N., Walbrun, A., Falleroni, F., Rief, M., and Sattler, M.

Conformational Effects of a Cancer-Linked Mutation in Pri-miR-30c RNA.

J Mol Biol 2022; 434: 167705

https://www.sciencedirect.com/science/article/pii/S0022283622002972?via%3Dihub

Singh, A., Rief, M., and Zoldak, G.

Direct observation of chemo-mechanical coupling in DnaK by single-molecule force experiments.

Biophys J 2022; 121: 4729-4739

https://www.cell.com/biophysj/fulltext/S0006-3495(22)00819-0

Synakewicz, M., Eapen, R.S., Perez-Riba, A., Rowling, P.J.E., Bauer, D., Weissl, A., Fischer, G., Hyvonen, M., Rief, M., Itzhaki, L.S., et al.

Unraveling the Mechanics of a Repeat-Protein Nanospring: From Folding of Individual Repeats to Fluctuations of the Superhelix.

ACS Nano 2022; 16: 3895-3905

https://pubs.acs.org/doi/10.1021/acsnano.1c09162

Tych, K., and Rief, M.

Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone.

Methods Mol Biol 2022; 2478: 401-425

https://link.springer.com/protocol/10.1007/978-1-0716-2229-2_15

2022
2021
2021

Sengupta, A., Rognoni, L.E., Merkel, U., Zoldak, G., and Rief, M.

SlyD Accelerates trans-to-cis Prolyl Isomerization in a Mechanosignaling Protein under Load.

J Phys Chem B

https://pubs.acs.org/doi/10.1021/acs.jpcb.1c03648

Sengupta, A., and Rief, M.

Energy landscapes of fast-folding proteins pushing the limits of atomic force microscope (AFM) pulling.

Proc Natl Acad Sci U S A 2021; 118

https://www.pnas.org/doi/10.1073/pnas.2102946118

2020
2020
2019
2019

Synakewicz, M.,Bauer, D.,Rief M.,Itzhaki, S.,

Bioorthogonal protein-DNA conjugation methods for force spectroscopy

Nature

https://www.nature.com/articles/s41598-019-49843-1

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Girstmair, H.,Tippel, F., Lopez A., Tych, K., Stein, F., Haberkant, P., Schmid, Ph., W., N., Helm, D., Rief, M., Sattler, M., Buchner, J.,

The Hsp90 isoforms from S. cerevisiae differ in structure, function and client range

Nature Communications

https://www.nature.com/articles/s41467-019-11518-w

​

Meinhold, S.,Bauer, D.,Huber, J.,Merkel U.,Weissl, A.,Zoldak, G. u. Rief, M.

An Active, Ligand-Responsive Pulling Geometry Reports on Internal Signaling between Subdomains of the DnaK Nucleotide-Binding Domain in Single-Molecule Mechanical Experiments

Biochemistry

https://doi.org/10.1021/acs.biochem.9b00155

​

2018
2018

Rognoni, L.;Most, T.;Zoldak, G.;Rief, M.

Force-dependent isomerization kinetics of a highly conserved proline switch modulates the mechanosensing region of filamin

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=24706888

Ramm, B.;Stigler, J.;Hinczewski, M.;Thirumalai, D.;Herrmann, H.;Woehlke, G.;Rief, M.

Sequence-resolved free energy profiles of stress-bearing vimentin intermediate filaments

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/pubmed/25049381

Jahn, M.;Rehn, A.;Pelz, B.;Hellenkamp, B.;Richter, K.;Rief, M.;Buchner, J.;Hugel, T.

The charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/pubmed/25468961

Jahn, M.;Tych, K.;Girstmair, H.;Steinmassl, M.;Hugel, T.;Buchner, J.;Rief, M.

Folding and Domain Interactions of Three Orthologs of Hsp90 Studied by Single-Molecule Force Spectroscopy

Structure

https://www.ncbi.nlm.nih.gov/pubmed/29276035

Bauer, D.;Meinhold, S.;Jakob, R. P.;Stigler, J.;Merkel, U.;Maier, T.;Rief, M.;Zoldak, G.

A folding nucleus and minimal ATP binding domain of Hsp70 identified by single-molecule force spectroscopy

Proc Natl Acad Sci U S A

https://www.ncbi.nlm.nih.gov/pubmed/29669923

Suren, T.;Rutz, D.;Mossmer, P.;Merkel, U.;Buchner, J.;Rief, M.

Single-molecule force spectroscopy reveals folding steps associated with hormone binding and activation of the glucocorticoid receptor

Proc Natl Acad Sci U S A

https://www.ncbi.nlm.nih.gov/pubmed/30366952

Tych, K. M.;Jahn, M.;Gegenfurtner, F.;Hechtl, V. K.;Buchner, J.;Hugel, T.;Rief, M.

Nucleotide-Dependent Dimer Association and Dissociation of the Chaperone Hsp90

J Phys Chem B

https://www.ncbi.nlm.nih.gov/pubmed/30179494

Tych,K.: Jahn,M.; Girstmair H.; Hugel, T.; Buchner, J.; Rief, M.

Unravelling the Mechanics of a Molecular Chaperone

Biophysical Journal 114 (3), 552a

https://www.cell.com/biophysj/fulltext/S0006-3495(17)34249-2

2017
2017

Ringer, P.;Weissl, A.;Cost, A. L.;Freikamp, A.;Sabass, B.;Mehlich, A.;Tramier, M.;Rief, M.;Grashoff, C.

Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1

Nat Methods

https://www.ncbi.nlm.nih.gov/pubmed/28945706

Ganim, Z.;Rief, M.

Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Proc Natl Acad Sci U S A

https://www.ncbi.nlm.nih.gov/pubmed/29073015

Rief, M.;Muller, D. J.

Special issue: Biomolecular forces

J Struct Biol

https://www.ncbi.nlm.nih.gov/pubmed/28061956

Mandal, S. S.;Merz, D. R.;Buchsteiner, M.;Dima, R. I.;Rief, M.;Zoldak, G.

Nanomechanics of the substrate binding domain of Hsp70 determine its allosteric ATP-induced conformational change

Proc Natl Acad Sci U S A

https://www.ncbi.nlm.nih.gov/pubmed/28533394

Grison, M.;Merkel, U.;Kostan, J.;Djinovic-Carugo, K.;Rief, M.

alpha-Actinin/titin interaction: A dynamic and mechanically stable cluster of bonds in the muscle Z-disk

Proc Natl Acad Sci U S A

https://www.ncbi.nlm.nih.gov/pubmed/28096424
2016
2016

Jahn, M.;Buchner, J.;Hugel, T.;Rief, M.

Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/pubmed/26787848

Pelz, B.;Zoldak, G.;Zeller, F.;Zacharias, M.;Rief, M.

Subnanometre enzyme mechanics probed by single-molecule force spectroscopy

Nat Commun

http://www.ncbi.nlm.nih.gov/pubmed/26906294

Kilchherr, F.;Wachauf, C.;Pelz, B.;Rief, M.;Zacharias, M.;Dietz, H.

Single-molecule dissection of stacking forces in DNA

Science

http://www.ncbi.nlm.nih.gov/pubmed/27609897

Ziegler, F.;Lim, N. C.;Mandal, S. S.;Pelz, B.;Ng, W. P.;Schlierf, M.;Jackson, S. E.;Rief, M.

Knotting and unknotting of a protein in single molecule experiments

Proc Natl Acad Sci U S A

https://www.ncbi.nlm.nih.gov/pubmed/27339135
2015
2015

Hocking, H. G.;Hase, F.;Madl, T.;Zacharias, M.;Rief, M.;Zoldak, G.

A Compact Native 24-Residue Supersecondary Structure Derived from the Villin Headpiece Subdomain

Biophys J

http://www.ncbi.nlm.nih.gov/pubmed/25650934

Bauer, D.;Merz, D. R.;Pelz, B.;Theisen, K. E.;Yacyshyn, G.;Mokranjac, D.;Dima, R. I.;Rief, M.;Zoldak, G.

Nucleotides regulate the mechanical hierarchy between subdomains of the nucleotide binding domain of the Hsp70 chaperone DnaK

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/pubmed/26240360

Stigler, J.;Rief, M.

Ligand-Induced Changes of the Apparent Transition-State Position in Mechanical Protein Unfolding

Biophys J

http://www.ncbi.nlm.nih.gov/pubmed/26200872

Austen, K.;Ringer, P.;Mehlich, A.;Chrostek-Grashoff, A.;Kluger, C.;Klingner, C.;Sabass, B.;Zent, R.;Rief, M.;Grashoff, C.

Extracellular rigidity sensing by talin isoform-specific mechanical linkages

Nat Cell Biol

http://www.ncbi.nlm.nih.gov/pubmed/26523364
2014
2014

Rognoni, L.;Most, T.;Zoldak, G.;Rief, M.

Force-dependent isomerization kinetics of a highly conserved proline switch modulates the mechanosensing region of filamin

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=24706888

Ramm, B.;Stigler, J.;Hinczewski, M.;Thirumalai, D.;Herrmann, H.;Woehlke, G.;Rief, M.

Sequence-resolved free energy profiles of stress-bearing vimentin intermediate filaments

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/pubmed/25049381

Jahn, M.;Rehn, A.;Pelz, B.;Hellenkamp, B.;Richter, K.;Rief, M.;Buchner, J.;Hugel, T.

The charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/pubmed/25468961
2013
2013

Hinczewski, M.;Gebhardt, J. C.;Rief, M.;Thirumalai, D.

From mechanical folding trajectories to intrinsic energy landscapes of biopolymers

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=23487746

Zoldak, G.;Rief, M.

Force as a single molecule probe of multidimensional protein energy landscapes

Curr Opin Struct Biol

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=23279960

Zoldak, G.;Stigler, J.;Pelz, B.;Li, H.;Rief, M.

Ultrafast folding kinetics and cooperativity of villin headpiece in single-molecule force spectroscopy

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=24145407

Fang, J.;Mehlich, A.;Koga, N.;Huang, J.;Koga, R.;Gao, X.;Hu, C.;Jin, C.;Rief, M.;Kast, J.;Baker, D.;Li, H.

Forced protein unfolding leads to highly elastic and tough protein hydrogels

Nat Commun

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=24352111

Pfitzner, E.;Wachauf, C.;Kilchherr, F.;Pelz, B.;Shih, W. M.;Rief, M.;Dietz, H.

Rigid DNA beams for high-resolution single-molecule mechanics

Angew Chem Int Ed Engl

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=23794413

Bertz, M.;Buchner, J.;Rief, M.

Mechanical stability of the antibody domain CH3 homodimer in different oxidation states

J Am Chem Soc

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=24015948
2012
2012

Pinotsis, N.;Chatziefthimiou, S. D.;Berkemeier, F.;Beuron, F.;Mavridis, I. M.;Konarev, P. V.;Svergun, D. I.;Morris, E.;Rief, M.;Wilmanns, M.

Superhelical architecture of the Myosin filament-linking protein myomesin with unusual elastic properties

PLoS Biol

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22347812

Brunnbauer, M.;Dombi, R.;Ho, T. H.;Schliwa, M.;Rief, M.;Okten, Z.

Torque generation of Kinesin motors is governed by the stability of the neck domain

Mol Cell

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22541555

von Hansen, Y.;Mehlich, A.;Pelz, B.;Rief, M.;Netz, R. R.

Auto- and cross-power spectral analysis of dual trap optical tweezer experiments using Bayesian inference

Rev Sci Instrum

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=23020428

Rognoni, L.;Stigler, J.;Pelz, B.;Ylanne, J.;Rief, M.

Dynamic force sensing of filamin revealed in single-molecule experiments

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=23150587

Stigler, J.;Rief, M.

Hidden Markov analysis of trajectories in single-molecule experiments and the effects of missed events

Chemphyschem

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22392881

Stigler, J.;Rief, M.

Calcium-dependent folding of single calmodulin molecules

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22753517
2011
2011

Berkemeier, F.;Bertz, M.;Xiao, S.;Pinotsis, N.;Wilmanns, M.;Grater, F.;Rief, M.

Fast-folding {alpha}-helices as reversible strain absorbers in the muscle protein myomesin

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21825161

Stigler, J.;Ziegler, F.;Gieseke, A.;Gebhardt, J. C.;Rief, M.

The complex folding network of single calmodulin molecules

Science

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22034433
2011
2011
2010 and older
2010 and older

Junker, J. P.;Rief, M.

Evidence for a broad transition-state ensemble in calmodulin folding from single-molecule force spectroscopy

Angew Chem Int Ed Engl

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20352635

Yew, Z. T.;Schlierf, M.;Rief, M.;Paci, E.

Direct evidence of the multidimensionality of the free-energy landscapes of proteins revealed by mechanical probes

Phys Rev E Stat Nonlin Soft Matter Phys

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20365786

Gebhardt, J. C. M.;Okten, Z.;Rief, M.

The Lever Arm Effects a Mechanical Asymmetry of the Myosin-V-Actin Bond

Biophysical Journal

https://pubmed.ncbi.nlm.nih.gov/20338849/

Gebhardt, J. C.;Bornschlogl, T.;Rief, M.

Full distance-resolved folding energy landscape of one single protein molecule

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20133846

Pernigo, S.;Fukuzawa, A.;Bertz, M.;Holt, M.;Rief, M.;Steiner, R. A.;Gautel, M.

Structural insight into M-band assembly and mechanics from the titin-obscurin-like-1 complex

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20133654

Brunnbauer, M.;Mueller-Planitz, F.;Kosem, S.;Ho, T. H.;Dombi, R.;Gebhardt, J. C.;Rief, M.;Okten, Z.

Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20498083

Bertz, M.;Chen, J.;Feige, M. J.;Franzmann, T. M.;Buchner, J.;Rief, M.

Structural and mechanical hierarchies in the alpha-crystallin domain dimer of the hyperthermophilic small heat shock protein Hsp16.5

J Mol Biol

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20595041

Schlierf, M.;Yew, Z. T.;Rief, M.;Paci, E.

Complex unfolding kinetics of single-domain proteins in the presence of force

Biophys J

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20816075

Bornschlogl, T.;Gebhardt, J. C.;Rief, M.

Designing the Folding Mechanics of Coiled Coils

Chemphyschem

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19746505

Bornschlogl, T.;Anstrom, D. M.;Mey, E.;Dzubiella, J.;Rief, M.;Forest, K. T.

Tightening the knot in phytochrome by single-molecule atomic force microscopy

Biophys J

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19217867

Schlierf, M.;Rief, M.

Surprising simplicity in the single-molecule folding mechanics of proteins

Angew Chem Int Ed Engl

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19101973

Bertz, M.;Wilmanns, M.;Rief, M.

The titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19622741

Gebhardt, JCM.;Rief, M.

Biochemistry. Force signaling in biology

Science

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19498156

Bertz, M.;Rief, M.

Ligand binding mechanics of maltose binding protein

J Mol Biol

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19733183

Adio, S.;Jaud, J.;Ebbing, B.;Rief, M.;Woehlke, G.

Dissection of Kinesin's Processivity

PLoS ONE

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19242550

Bornschlogl, T.;Woehlke, G.;Rief, M.

Single molecule mechanics of the kinesin neck

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19369199

Junker, J. P.;Rief, M.

Single-molecule force spectroscopy distinguishes target binding modes of calmodulin

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19667195

Junker, J. P.;Ziegler, F.;Rief, M.

Ligand-dependent equilibrium fluctuations of single calmodulin molecules

Science

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19179531

Bertz, M.;Rief, M.

Mechanical Unfoldons as Building Blocks of Maltose-binding Protein

J Mol Biol

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18355837

Dietz, H.;Rief, M.

Elastic bond network model for protein unfolding mechanics

Phys Rev Lett

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18352751

Bertz, M.;Kunfermann, A.;Rief, M.

Navigating the folding energy landscape of green fluorescent protein

Angew Chem Int Ed Engl

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18785668

Bornschlogl, T.;Rief, M.

Single-molecule dynamics of mechanical coiled-coil unzipping

Langmuir

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17973511

Cappello, G.;Pierobon, P.;Symonds, C.;Busoni, L.;Gebhardt, J. C.;Rief, M.;Prost, J.

Myosin V stepping mechanism

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17878301

Dietz, H.;Bornschlogl, T.;Heym, R.;Konig, F.;Rief, M.

Programming protein self assembly with coiled coils

New Journal of Physics

://000251533800009

Schlierf, M.;Berkemeier, F.;Rief, M.

Direct Observation of Active Protein Folding Using Lock-in Force Spectroscopy

Biophys J

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17704164

Mickler, M.;Dima, R. I.;Dietz, H.;Hyeon, C.;Thirumalai, D.;Rief, M.

Revealing the bifurcation in the unfolding pathways of GFP by using single-molecule experiments and simulations

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18079292

Khaire, N.;Muller, R.;Blau-Wasser, R.;Eichinger, L.;Schleicher, M.;Rief, M.;Holak, T. A.;Noegel, A. A.

Filamin-regulated F-actin assembly is essential for morphogenesis and controls phototaxis in Dictyostelium

J Biol Chem

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17121815

Dietz, H.;Rief, M.

Detecting molecular fingerprints in single molecule force spectroscopy using pattern recognition

Japanese Journal of Applied Physics Part 1-Regular Papers Brief Communications & Review Papers

://000249219200029

Jaud, J.;Bathe, F.;Schliwa, M.;Rief, M.;Woehlke, G.

Flexibility of the neck domain enhances Kinesin-1 motility under load

Biophys J

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16714343

Gebhardt, J. C.;Clemen, A. E.;Jaud, J.;Rief, M.

Myosin-V is a mechanical ratchet

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16731631

Schlierf, M.;Rief, M.

Single-molecule unfolding force distributions reveal a funnel-shaped energy landscape

Biophys J

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16361331

Rief, M.;Junker, J. P.;Schlierf, M.;Hell, K.;Neupert, W.

Response to the Comment by Ainavarapu et al

Biophys J

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16782790

Dietz, H.;Bertz, M.;Schlierf, M.;Berkemeier, F.;Bornschlogl, T.;Junker, J. P.;Rief, M.

Cysteine engineering of polyproteins for single-molecule force spectroscopy

Nat Protoc

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17406215

Berkemeier, F.;Schlierf, M.;Rief, M.

Mechanically controlled preparation of protein intermediates in single molecule experiments

PHYSICA STATUS SOLIDI A

https://onlinelibrary.wiley.com/doi/abs/10.1002/pssa.200622384

Bornschlogl, T.;Rief, M.

Single molecule unzipping of coiled coils: sequence resolved stability profiles

Phys Rev Lett

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16605876

Dietz, H.;Berkemeier, F.;Bertz, M.;Rief, M.

Anisotropic deformation response of single protein molecules

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16908850

Dietz, H.;Rief, M.

Protein structure by mechanical triangulation

Proc Natl Acad Sci U S A

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16432239

Junker, J. P.;Hell, K.;Schlierf, M.;Neupert, W.;Rief, M.

Influence of substrate binding on the mechanical stability of mouse dihydrofolate reductase

Biophysical Journal

://000232933300006

Hugel, T.;Rief, M.;Seitz, M.;Gaub, H. E.;Netz, R. R.

Highly stretched single polymers: Atomic-force-microscope experiments versus ab-initio theory

Physical Review Letters

://000226779000074

Kufer, S. K.;Dietz, H.;Albrecht, C.;Blank, K.;Kardinal, A.;Rief, M.;Gaub, H. E.

Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy

European Biophysics Journal with Biophysics Letters

://000233392200006

Schwaiger, I.;Schleicher, M.;Noegel, A. A.;Rief, M.

The folding pathway of a fast-folding immunoglobulin domain revealed by single-molecule mechanical experiments

Embo Reports

://000226183500011

Schlierf, M.;Rief, M.

Temperature Softening of a Protein in Single-molecule Experiments

J Mol Biol

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16246362

Clemen, A. E. M.;Vilfan, M.;Jaud, J.;Zhang, J. S.;Barmann, M.;Rief, M.

Force-dependent stepping kinetics of myosin-V

Biophysical Journal

://000229503000064

Schwaiger, I.;Kardinal, A.;Schleicher, M.;Noegel, A. A.;Rief, M.

A mechanical unfolding intermediate in an actin-crosslinking protein

Nature Structural & Molecular Biology

://000220143900017

Dietz, H.;Rief, M.

Exploring the energy landscape of GFP by single-molecule mechanical experiments

Proc Natl Acad Sci U S A

://000225226200021

Zhuang, X. W.;Rief, M.

Single-molecule folding

Current Opinion in Structural Biology

://000181133300013

Krautbauer, R.;Rief, M.;Gaub, H. E.

Unzipping DNA oligomers

Nano Letters

://000182201800013

Schwaiger, I.;Sattler, C.;Hostetter, D. R.;Rief, M.

The myosin coiled-coil is a truly elastic protein structure

Nature Materials

://000181498800020

Rief, M.;Grubmuller, H.

Force spectroscopy of single biomolecules

Chemphyschem

://000174557400004

Clausen-Schaumann, H.;Rief, M.;Seitz, M.

Artificial noses sniff DNA

Chemphyschem

://000168279900002
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