Publications 

2019

Synakewicz, M.,Bauer, D.,Rief M.,Itzhaki, S.,

Bioorthogonal protein-DNA conjugation methods for force spectroscopy

Nature

Girstmair, H.,Tippel, F., Lopez A., Tych, K., Stein, F., Haberkant, P., Schmid, Ph., W., N., Helm, D., Rief, M., Sattler, M., Buchner, J.,

The Hsp90 isoforms from S. cerevisiae differ in structure, function and client range

Nature Communications

Meinhold, S.,Bauer, D.,Huber, J.,Merkel U.,Weissl, A.,Zoldak, G. u. Rief, M.

An Active, Ligand-Responsive Pulling Geometry Reports on Internal Signaling between Subdomains of the DnaK Nucleotide-Binding Domain in Single-Molecule Mechanical Experiments

Biochemistry

2018

Jahn, M.;Tych, K.;Girstmair, H.;Steinmassl, M.;Hugel, T.;Buchner, J.;Rief, M.

Folding and Domain Interactions of Three Orthologs of Hsp90 Studied by Single-Molecule Force Spectroscopy

Structure

Bauer, D.;Meinhold, S.;Jakob, R. P.;Stigler, J.;Merkel, U.;Maier, T.;Rief, M.;Zoldak, G.

A folding nucleus and minimal ATP binding domain of Hsp70 identified by single-molecule force spectroscopy

Proc Natl Acad Sci U S A

Suren, T.;Rutz, D.;Mossmer, P.;Merkel, U.;Buchner, J.;Rief, M.

Single-molecule force spectroscopy reveals folding steps associated with hormone binding and activation of the glucocorticoid receptor

Proc Natl Acad Sci U S A

Tych, K. M.;Jahn, M.;Gegenfurtner, F.;Hechtl, V. K.;Buchner, J.;Hugel, T.;Rief, M.

Nucleotide-Dependent Dimer Association and Dissociation of the Chaperone Hsp90

J Phys Chem B

 
2017

Ringer, P.;Weissl, A.;Cost, A. L.;Freikamp, A.;Sabass, B.;Mehlich, A.;Tramier, M.;Rief, M.;Grashoff, C.

Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1

Nat Methods

Ganim, Z.;Rief, M.

Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Proc Natl Acad Sci U S A

Rief, M.;Muller, D. J.

Special issue: Biomolecular forces

J Struct Biol

Mandal, S. S.;Merz, D. R.;Buchsteiner, M.;Dima, R. I.;Rief, M.;Zoldak, G.

Nanomechanics of the substrate binding domain of Hsp70 determine its allosteric ATP-induced conformational change

Proc Natl Acad Sci U S A

Grison, M.;Merkel, U.;Kostan, J.;Djinovic-Carugo, K.;Rief, M.

alpha-Actinin/titin interaction: A dynamic and mechanically stable cluster of bonds in the muscle Z-disk

Proc Natl Acad Sci U S A

 
2016

Jahn, M.;Buchner, J.;Hugel, T.;Rief, M.

Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments

Proc Natl Acad Sci U S A

Pelz, B.;Zoldak, G.;Zeller, F.;Zacharias, M.;Rief, M.

Subnanometre enzyme mechanics probed by single-molecule force spectroscopy

Nat Commun

Kilchherr, F.;Wachauf, C.;Pelz, B.;Rief, M.;Zacharias, M.;Dietz, H.

Single-molecule dissection of stacking forces in DNA

Science

Ziegler, F.;Lim, N. C.;Mandal, S. S.;Pelz, B.;Ng, W. P.;Schlierf, M.;Jackson, S. E.;Rief, M.

Knotting and unknotting of a protein in single molecule experiments

Proc Natl Acad Sci U S A

 
2015

Hocking, H. G.;Hase, F.;Madl, T.;Zacharias, M.;Rief, M.;Zoldak, G.

A Compact Native 24-Residue Supersecondary Structure Derived from the Villin Headpiece Subdomain

Biophys J

Bauer, D.;Merz, D. R.;Pelz, B.;Theisen, K. E.;Yacyshyn, G.;Mokranjac, D.;Dima, R. I.;Rief, M.;Zoldak, G.

Nucleotides regulate the mechanical hierarchy between subdomains of the nucleotide binding domain of the Hsp70 chaperone DnaK

Proc Natl Acad Sci U S A

Stigler, J.;Rief, M.

Ligand-Induced Changes of the Apparent Transition-State Position in Mechanical Protein Unfolding

Biophys J

Austen, K.;Ringer, P.;Mehlich, A.;Chrostek-Grashoff, A.;Kluger, C.;Klingner, C.;Sabass, B.;Zent, R.;Rief, M.;Grashoff, C.

Extracellular rigidity sensing by talin isoform-specific mechanical linkages

Nat Cell Biol

 
2014

Rognoni, L.;Most, T.;Zoldak, G.;Rief, M.

Force-dependent isomerization kinetics of a highly conserved proline switch modulates the mechanosensing region of filamin

Proc Natl Acad Sci U S A

Ramm, B.;Stigler, J.;Hinczewski, M.;Thirumalai, D.;Herrmann, H.;Woehlke, G.;Rief, M.

Sequence-resolved free energy profiles of stress-bearing vimentin intermediate filaments

Proc Natl Acad Sci U S A

Jahn, M.;Rehn, A.;Pelz, B.;Hellenkamp, B.;Richter, K.;Rief, M.;Buchner, J.;Hugel, T.

The charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function

Proc Natl Acad Sci U S A

 
2013

Hinczewski, M.;Gebhardt, J. C.;Rief, M.;Thirumalai, D.

From mechanical folding trajectories to intrinsic energy landscapes of biopolymers

Proc Natl Acad Sci U S A

Zoldak, G.;Rief, M.

Force as a single molecule probe of multidimensional protein energy landscapes

Curr Opin Struct Biol

Zoldak, G.;Stigler, J.;Pelz, B.;Li, H.;Rief, M.

Ultrafast folding kinetics and cooperativity of villin headpiece in single-molecule force spectroscopy

Proc Natl Acad Sci U S A

Fang, J.;Mehlich, A.;Koga, N.;Huang, J.;Koga, R.;Gao, X.;Hu, C.;Jin, C.;Rief, M.;Kast, J.;Baker, D.;Li, H.

Forced protein unfolding leads to highly elastic and tough protein hydrogels

Nat Commun

Pfitzner, E.;Wachauf, C.;Kilchherr, F.;Pelz, B.;Shih, W. M.;Rief, M.;Dietz, H.

Rigid DNA beams for high-resolution single-molecule mechanics

Angew Chem Int Ed Engl

Bertz, M.;Buchner, J.;Rief, M.

Mechanical stability of the antibody domain CH3 homodimer in different oxidation states

J Am Chem Soc

 
2012

Pinotsis, N.;Chatziefthimiou, S. D.;Berkemeier, F.;Beuron, F.;Mavridis, I. M.;Konarev, P. V.;Svergun, D. I.;Morris, E.;Rief, M.;Wilmanns, M.

Publication Title

Proc Natl Acad Sci U S A

Brunnbauer, M.;Dombi, R.;Ho, T. H.;Schliwa, M.;Rief, M.;Okten, Z.

Publication Title

Proc Natl Acad Sci U S A

von Hansen, Y.;Mehlich, A.;Pelz, B.;Rief, M.;Netz, R. R.

Publication Title

Proc Natl Acad Sci U S A

Rognoni, L.;Stigler, J.;Pelz, B.;Ylanne, J.;Rief, M.

Publication Title

Proc Natl Acad Sci U S A

 
2011
 

Berkemeier, F.;Bertz, M.;Xiao, S.;Pinotsis, N.;Wilmanns, M.;Grater, F.;Rief, M.

Fast-folding {alpha}-helices as reversible strain absorbers in the muscle protein myomesin

Proc Natl Acad Sci U S A

Stigler, J.;Ziegler, F.;Gieseke, A.;Gebhardt, J. C.;Rief, M.

The complex folding network of single calmodulin molecules

Science

2010 and older

Gebhardt, J. C. M.;Okten, Z.;Rief, M.

The Lever Arm Effects a Mechanical Asymmetry of the Myosin-V-Actin Bond

Biophysical Journal

[removed]://000273972700011

Junker, J. P.;Rief, M.

Evidence for a broad transition-state ensemble in calmodulin folding from single-molecule force spectroscopy

Angew Chem Int Ed Engl

Yew, Z. T.;Schlierf, M.;Rief, M.;Paci, E.

Direct evidence of the multidimensionality of the free-energy landscapes of proteins revealed by mechanical probes

Phys Rev E Stat Nonlin Soft Matter Phys

Bertz, M.;Chen, J.;Feige, M. J.;Franzmann, T. M.;Buchner, J.;Rief, M.

Structural and mechanical hierarchies in the alpha-crystallin domain dimer of the hyperthermophilic small heat shock protein Hsp16.5

J Mol Biol

Brunnbauer, M.;Mueller-Planitz, F.;Kosem, S.;Ho, T. H.;Dombi, R.;Gebhardt, J. C.;Rief, M.;Okten, Z.

Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner

Proc Natl Acad Sci U S A

Pernigo, S.;Fukuzawa, A.;Bertz, M.;Holt, M.;Rief, M.;Steiner, R. A.;Gautel, M.

Structural insight into M-band assembly and mechanics from the titin-obscurin-like-1 complex

Proc Natl Acad Sci U S A

Gebhardt, J. C.;Bornschlogl, T.;Rief, M.

Full distance-resolved folding energy landscape of one single protein molecule

Proc Natl Acad Sci U S A

Schlierf, M.;Yew, Z. T.;Rief, M.;Paci, E.

Complex unfolding kinetics of single-domain proteins in the presence of force

Biophys J

Junker, J. P.;Ziegler, F.;Rief, M.

Ligand-dependent equilibrium fluctuations of single calmodulin molecules

Science

Bertz, M.;Wilmanns, M.;Rief, M.

The titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk

Proc Natl Acad Sci U S A

Bornschlogl, T.;Anstrom, D. M.;Mey, E.;Dzubiella, J.;Rief, M.;Forest, K. T.

Tightening the knot in phytochrome by single-molecule atomic force microscopy

Biophys J

Bornschlogl, T.;Gebhardt, J. C.;Rief, M.

Designing the Folding Mechanics of Coiled Coils

Chemphyschem

Schlierf, M.;Rief, M.

Surprising simplicity in the single-molecule folding mechanics of proteins

Angew Chem Int Ed Engl

Gebhardt, JCM.;Rief, M.

Biochemistry. Force signaling in biology

Science

Junker, J. P.;Rief, M.

Single-molecule force spectroscopy distinguishes target binding modes of calmodulin

Proc Natl Acad Sci U S A

Bertz, M.;Rief, M.

Ligand binding mechanics of maltose binding protein

J Mol Biol

Bornschlogl, T.;Woehlke, G.;Rief, M.

Single molecule mechanics of the kinesin neck

Proc Natl Acad Sci U S A

Adio, S.;Jaud, J.;Ebbing, B.;Rief, M.;Woehlke, G.

Dissection of Kinesin's Processivity

PLoS ONE

Bertz, M.;Rief, M.

Mechanical Unfoldons as Building Blocks of Maltose-binding Protein

J Mol Biol

Dietz, H.;Rief, M.

Elastic bond network model for protein unfolding mechanics

Phys Rev Lett

Bornschlogl, T.;Rief, M.

Single-molecule dynamics of mechanical coiled-coil unzipping

Langmuir

Bertz, M.;Kunfermann, A.;Rief, M.

Navigating the folding energy landscape of green fluorescent protein

Angew Chem Int Ed Engl

Dietz, H.;Rief, M.

Detecting molecular fingerprints in single molecule force spectroscopy using pattern recognition

Japanese Journal of Applied Physics Part 1-Regular Papers Brief Communications & Review Papers

[removed]://000249219200029

Schlierf, M.;Berkemeier, F.;Rief, M.

Direct Observation of Active Protein Folding Using Lock-in Force Spectroscopy

Biophys J

Mickler, M.;Dima, R. I.;Dietz, H.;Hyeon, C.;Thirumalai, D.;Rief, M.

Revealing the bifurcation in the unfolding pathways of GFP by using single-molecule experiments and simulations

Proc Natl Acad Sci U S A

Cappello, G.;Pierobon, P.;Symonds, C.;Busoni, L.;Gebhardt, J. C.;Rief, M.;Prost, J.

Myosin V stepping mechanism

Proc Natl Acad Sci U S A

Khaire, N.;Muller, R.;Blau-Wasser, R.;Eichinger, L.;Schleicher, M.;Rief, M.;Holak, T. A.;Noegel, A. A.

Filamin-regulated F-actin assembly is essential for morphogenesis and controls phototaxis in Dictyostelium

J Biol Chem

Dietz, H.;Bornschlogl, T.;Heym, R.;Konig, F.;Rief, M.

Programming protein self assembly with coiled coils

New Journal of Physics

[removed]://000251533800009

Schlierf, M.;Rief, M.

Single-molecule unfolding force distributions reveal a funnel-shaped energy landscape

Biophys J

Dietz, H.;Berkemeier, F.;Bertz, M.;Rief, M.

Anisotropic deformation response of single protein molecules

Proc Natl Acad Sci U S A

Rief, M.;Junker, J. P.;Schlierf, M.;Hell, K.;Neupert, W.

Response to the Comment by Ainavarapu et al

Biophys J

Gebhardt, J. C.;Clemen, A. E.;Jaud, J.;Rief, M.

Myosin-V is a mechanical ratchet

Proc Natl Acad Sci U S A

Berkemeier, F.;Schlierf, M.;Rief, M.

Mechanically controlled preparation of protein intermediates in single molecule experiments

PHYSICA STATUS SOLIDI A

Dietz, H.;Rief, M.

Protein structure by mechanical triangulation

Proc Natl Acad Sci U S A

Bornschlogl, T.;Rief, M.

Single molecule unzipping of coiled coils: sequence resolved stability profiles

Phys Rev Lett

Jaud, J.;Bathe, F.;Schliwa, M.;Rief, M.;Woehlke, G.

Flexibility of the neck domain enhances Kinesin-1 motility under load

Biophys J

Dietz, H.;Bertz, M.;Schlierf, M.;Berkemeier, F.;Bornschlogl, T.;Junker, J. P.;Rief, M.

Cysteine engineering of polyproteins for single-molecule force spectroscopy

Nat Protoc

Clemen, A. E. M.;Vilfan, M.;Jaud, J.;Zhang, J. S.;Barmann, M.;Rief, M.

Force-dependent stepping kinetics of myosin-V

Biophysical Journal

[removed]://000229503000064

Junker, J. P.;Hell, K.;Schlierf, M.;Neupert, W.;Rief, M.

Influence of substrate binding on the mechanical stability of mouse dihydrofolate reductase

Biophysical Journal

[removed]://000232933300006

Schlierf, M.;Rief, M.

Temperature Softening of a Protein in Single-molecule Experiments

J Mol Biol

Schwaiger, I.;Schleicher, M.;Noegel, A. A.;Rief, M.

The folding pathway of a fast-folding immunoglobulin domain revealed by single-molecule mechanical experiments

Embo Reports

[removed]://000226183500011

Hugel, T.;Rief, M.;Seitz, M.;Gaub, H. E.;Netz, R. R.

Highly stretched single polymers: Atomic-force-microscope experiments versus ab-initio theory

Physical Review Letters

[removed]://000226779000074

Kufer, S. K.;Dietz, H.;Albrecht, C.;Blank, K.;Kardinal, A.;Rief, M.;Gaub, H. E.

Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy

European Biophysics Journal with Biophysics Letters

[removed]://000233392200006

Dietz, H.;Rief, M.

Exploring the energy landscape of GFP by single-molecule mechanical experiments

Proc Natl Acad Sci U S A

[removed]://000225226200021

Schwaiger, I.;Kardinal, A.;Schleicher, M.;Noegel, A. A.;Rief, M.

A mechanical unfolding intermediate in an actin-crosslinking protein

Nature Structural & Molecular Biology

[removed]://000220143900017

Krautbauer, R.;Rief, M.;Gaub, H. E.

Unzipping DNA oligomers

Nano Letters

[removed]://000182201800013

Zhuang, X. W.;Rief, M.

Single-molecule folding

Current Opinion in Structural Biology

[removed]://000181133300013

Schwaiger, I.;Sattler, C.;Hostetter, D. R.;Rief, M.

The myosin coiled-coil is a truly elastic protein structure

Nature Materials

[removed]://000181498800020

Rief, M.;Grubmuller, H.

Force spectroscopy of single biomolecules

Chemphyschem

[removed]://000174557400004

Rief, M.;Gautel, M.;Gaub, H. E.

Unfolding forces of titin and fibronectin domains directly measured by AFM

Elastic Filaments of the Cell

[removed]://000167396000008