Publications 

2018

Jahn, M.;Tych, K.;Girstmair, H.;Steinmassl, M.;Hugel, T.;Buchner, J.;Rief, M.

Folding and Domain Interactions of Three Orthologs of Hsp90 Studied by Single-Molecule Force Spectroscopy

Structure

Bauer, D.;Meinhold, S.;Jakob, R. P.;Stigler, J.;Merkel, U.;Maier, T.;Rief, M.;Zoldak, G.

A folding nucleus and minimal ATP binding domain of Hsp70 identified by single-molecule force spectroscopy

Proc Natl Acad Sci U S A

Suren, T.;Rutz, D.;Mossmer, P.;Merkel, U.;Buchner, J.;Rief, M.

Single-molecule force spectroscopy reveals folding steps associated with hormone binding and activation of the glucocorticoid receptor

Proc Natl Acad Sci U S A

Tych, K. M.;Jahn, M.;Gegenfurtner, F.;Hechtl, V. K.;Buchner, J.;Hugel, T.;Rief, M.

Nucleotide-Dependent Dimer Association and Dissociation of the Chaperone Hsp90

J Phys Chem B

 
2017

Ringer, P.;Weissl, A.;Cost, A. L.;Freikamp, A.;Sabass, B.;Mehlich, A.;Tramier, M.;Rief, M.;Grashoff, C.

Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1

Nat Methods

Ganim, Z.;Rief, M.

Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Proc Natl Acad Sci U S A

Rief, M.;Muller, D. J.

Special issue: Biomolecular forces

J Struct Biol

Mandal, S. S.;Merz, D. R.;Buchsteiner, M.;Dima, R. I.;Rief, M.;Zoldak, G.

Nanomechanics of the substrate binding domain of Hsp70 determine its allosteric ATP-induced conformational change

Proc Natl Acad Sci U S A

Grison, M.;Merkel, U.;Kostan, J.;Djinovic-Carugo, K.;Rief, M.

alpha-Actinin/titin interaction: A dynamic and mechanically stable cluster of bonds in the muscle Z-disk

Proc Natl Acad Sci U S A

 
2016

Jahn, M.;Buchner, J.;Hugel, T.;Rief, M.

Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments

Proc Natl Acad Sci U S A

Pelz, B.;Zoldak, G.;Zeller, F.;Zacharias, M.;Rief, M.

Subnanometre enzyme mechanics probed by single-molecule force spectroscopy

Nat Commun

Kilchherr, F.;Wachauf, C.;Pelz, B.;Rief, M.;Zacharias, M.;Dietz, H.

Single-molecule dissection of stacking forces in DNA

Science

Ziegler, F.;Lim, N. C.;Mandal, S. S.;Pelz, B.;Ng, W. P.;Schlierf, M.;Jackson, S. E.;Rief, M.

Knotting and unknotting of a protein in single molecule experiments

Proc Natl Acad Sci U S A

 
2015

Hocking, H. G.;Hase, F.;Madl, T.;Zacharias, M.;Rief, M.;Zoldak, G.

A Compact Native 24-Residue Supersecondary Structure Derived from the Villin Headpiece Subdomain

Biophys J

Bauer, D.;Merz, D. R.;Pelz, B.;Theisen, K. E.;Yacyshyn, G.;Mokranjac, D.;Dima, R. I.;Rief, M.;Zoldak, G.

Nucleotides regulate the mechanical hierarchy between subdomains of the nucleotide binding domain of the Hsp70 chaperone DnaK

Proc Natl Acad Sci U S A

Stigler, J.;Rief, M.

Ligand-Induced Changes of the Apparent Transition-State Position in Mechanical Protein Unfolding

Biophys J

Austen, K.;Ringer, P.;Mehlich, A.;Chrostek-Grashoff, A.;Kluger, C.;Klingner, C.;Sabass, B.;Zent, R.;Rief, M.;Grashoff, C.

Extracellular rigidity sensing by talin isoform-specific mechanical linkages

Nat Cell Biol

 
2014

Rognoni, L.;Most, T.;Zoldak, G.;Rief, M.

Force-dependent isomerization kinetics of a highly conserved proline switch modulates the mechanosensing region of filamin

Proc Natl Acad Sci U S A

Ramm, B.;Stigler, J.;Hinczewski, M.;Thirumalai, D.;Herrmann, H.;Woehlke, G.;Rief, M.

Sequence-resolved free energy profiles of stress-bearing vimentin intermediate filaments

Proc Natl Acad Sci U S A

Jahn, M.;Rehn, A.;Pelz, B.;Hellenkamp, B.;Richter, K.;Rief, M.;Buchner, J.;Hugel, T.

The charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function

Proc Natl Acad Sci U S A

 
2013

Hinczewski, M.;Gebhardt, J. C.;Rief, M.;Thirumalai, D.

From mechanical folding trajectories to intrinsic energy landscapes of biopolymers

Proc Natl Acad Sci U S A

Zoldak, G.;Rief, M.

Force as a single molecule probe of multidimensional protein energy landscapes

Curr Opin Struct Biol

Zoldak, G.;Stigler, J.;Pelz, B.;Li, H.;Rief, M.

Ultrafast folding kinetics and cooperativity of villin headpiece in single-molecule force spectroscopy

Proc Natl Acad Sci U S A

Fang, J.;Mehlich, A.;Koga, N.;Huang, J.;Koga, R.;Gao, X.;Hu, C.;Jin, C.;Rief, M.;Kast, J.;Baker, D.;Li, H.

Forced protein unfolding leads to highly elastic and tough protein hydrogels

Nat Commun

Pfitzner, E.;Wachauf, C.;Kilchherr, F.;Pelz, B.;Shih, W. M.;Rief, M.;Dietz, H.

Rigid DNA beams for high-resolution single-molecule mechanics

Angew Chem Int Ed Engl

Bertz, M.;Buchner, J.;Rief, M.

Mechanical stability of the antibody domain CH3 homodimer in different oxidation states

J Am Chem Soc

 
2012

Pinotsis, N.;Chatziefthimiou, S. D.;Berkemeier, F.;Beuron, F.;Mavridis, I. M.;Konarev, P. V.;Svergun, D. I.;Morris, E.;Rief, M.;Wilmanns, M.

Publication Title

Proc Natl Acad Sci U S A

Brunnbauer, M.;Dombi, R.;Ho, T. H.;Schliwa, M.;Rief, M.;Okten, Z.

Publication Title

Proc Natl Acad Sci U S A

von Hansen, Y.;Mehlich, A.;Pelz, B.;Rief, M.;Netz, R. R.

Publication Title

Proc Natl Acad Sci U S A

Rognoni, L.;Stigler, J.;Pelz, B.;Ylanne, J.;Rief, M.

Publication Title

Proc Natl Acad Sci U S A

 
2011
 

Berkemeier, F.;Bertz, M.;Xiao, S.;Pinotsis, N.;Wilmanns, M.;Grater, F.;Rief, M.

Fast-folding {alpha}-helices as reversible strain absorbers in the muscle protein myomesin

Proc Natl Acad Sci U S A

Stigler, J.;Ziegler, F.;Gieseke, A.;Gebhardt, J. C.;Rief, M.

The complex folding network of single calmodulin molecules

Science

2010 and older

Brunnbauer, M.;Mueller-Planitz, F.;Kosem, S.;Ho, T. H.;Dombi, R.;Gebhardt, J. C.;Rief, M.;Okten, Z.

Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner

Proc Natl Acad Sci U S A

Yew, Z. T.;Schlierf, M.;Rief, M.;Paci, E.

Direct evidence of the multidimensionality of the free-energy landscapes of proteins revealed by mechanical probes

Phys Rev E Stat Nonlin Soft Matter Phys

Bertz, M.;Chen, J.;Feige, M. J.;Franzmann, T. M.;Buchner, J.;Rief, M.

Structural and mechanical hierarchies in the alpha-crystallin domain dimer of the hyperthermophilic small heat shock protein Hsp16.5

J Mol Biol

Junker, J. P.;Rief, M.

Evidence for a broad transition-state ensemble in calmodulin folding from single-molecule force spectroscopy

Angew Chem Int Ed Engl

Gebhardt, J. C.;Bornschlogl, T.;Rief, M.

Full distance-resolved folding energy landscape of one single protein molecule

Proc Natl Acad Sci U S A

Schlierf, M.;Yew, Z. T.;Rief, M.;Paci, E.

Complex unfolding kinetics of single-domain proteins in the presence of force

Biophys J

Gebhardt, J. C. M.;Okten, Z.;Rief, M.

The Lever Arm Effects a Mechanical Asymmetry of the Myosin-V-Actin Bond

Biophysical Journal

[removed]://000273972700011

Pernigo, S.;Fukuzawa, A.;Bertz, M.;Holt, M.;Rief, M.;Steiner, R. A.;Gautel, M.

Structural insight into M-band assembly and mechanics from the titin-obscurin-like-1 complex

Proc Natl Acad Sci U S A

Schlierf, M.;Rief, M.

Surprising simplicity in the single-molecule folding mechanics of proteins

Angew Chem Int Ed Engl

Junker, J. P.;Ziegler, F.;Rief, M.

Ligand-dependent equilibrium fluctuations of single calmodulin molecules

Science

Gebhardt, JCM.;Rief, M.

Biochemistry. Force signaling in biology

Science

Bertz, M.;Rief, M.

Ligand binding mechanics of maltose binding protein

J Mol Biol

Bornschlogl, T.;Woehlke, G.;Rief, M.

Single molecule mechanics of the kinesin neck

Proc Natl Acad Sci U S A

Bornschlogl, T.;Anstrom, D. M.;Mey, E.;Dzubiella, J.;Rief, M.;Forest, K. T.

Tightening the knot in phytochrome by single-molecule atomic force microscopy

Biophys J

Bertz, M.;Wilmanns, M.;Rief, M.

The titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk

Proc Natl Acad Sci U S A

Bornschlogl, T.;Gebhardt, J. C.;Rief, M.

Designing the Folding Mechanics of Coiled Coils

Chemphyschem

Junker, J. P.;Rief, M.

Single-molecule force spectroscopy distinguishes target binding modes of calmodulin

Proc Natl Acad Sci U S A

Adio, S.;Jaud, J.;Ebbing, B.;Rief, M.;Woehlke, G.

Dissection of Kinesin's Processivity

PLoS ONE

Bertz, M.;Rief, M.

Mechanical Unfoldons as Building Blocks of Maltose-binding Protein

J Mol Biol

Bertz, M.;Kunfermann, A.;Rief, M.

Navigating the folding energy landscape of green fluorescent protein

Angew Chem Int Ed Engl

Dietz, H.;Rief, M.

Elastic bond network model for protein unfolding mechanics

Phys Rev Lett

Bornschlogl, T.;Rief, M.

Single-molecule dynamics of mechanical coiled-coil unzipping

Langmuir

Khaire, N.;Muller, R.;Blau-Wasser, R.;Eichinger, L.;Schleicher, M.;Rief, M.;Holak, T. A.;Noegel, A. A.

Filamin-regulated F-actin assembly is essential for morphogenesis and controls phototaxis in Dictyostelium

J Biol Chem

Dietz, H.;Rief, M.

Detecting molecular fingerprints in single molecule force spectroscopy using pattern recognition

Japanese Journal of Applied Physics Part 1-Regular Papers Brief Communications & Review Papers

[removed]://000249219200029

Cappello, G.;Pierobon, P.;Symonds, C.;Busoni, L.;Gebhardt, J. C.;Rief, M.;Prost, J.

Myosin V stepping mechanism

Proc Natl Acad Sci U S A

Mickler, M.;Dima, R. I.;Dietz, H.;Hyeon, C.;Thirumalai, D.;Rief, M.

Revealing the bifurcation in the unfolding pathways of GFP by using single-molecule experiments and simulations

Proc Natl Acad Sci U S A

Dietz, H.;Bornschlogl, T.;Heym, R.;Konig, F.;Rief, M.

Programming protein self assembly with coiled coils

New Journal of Physics

[removed]://000251533800009

Schlierf, M.;Berkemeier, F.;Rief, M.

Direct Observation of Active Protein Folding Using Lock-in Force Spectroscopy

Biophys J

Berkemeier, F.;Schlierf, M.;Rief, M.

Mechanically controlled preparation of protein intermediates in single molecule experiments

PHYSICA STATUS SOLIDI A

Gebhardt, J. C.;Clemen, A. E.;Jaud, J.;Rief, M.

Myosin-V is a mechanical ratchet

Proc Natl Acad Sci U S A

Dietz, H.;Berkemeier, F.;Bertz, M.;Rief, M.

Anisotropic deformation response of single protein molecules

Proc Natl Acad Sci U S A

Jaud, J.;Bathe, F.;Schliwa, M.;Rief, M.;Woehlke, G.

Flexibility of the neck domain enhances Kinesin-1 motility under load

Biophys J

Rief, M.;Junker, J. P.;Schlierf, M.;Hell, K.;Neupert, W.

Response to the Comment by Ainavarapu et al

Biophys J

Dietz, H.;Bertz, M.;Schlierf, M.;Berkemeier, F.;Bornschlogl, T.;Junker, J. P.;Rief, M.

Cysteine engineering of polyproteins for single-molecule force spectroscopy

Nat Protoc

Bornschlogl, T.;Rief, M.

Single molecule unzipping of coiled coils: sequence resolved stability profiles

Phys Rev Lett

Dietz, H.;Rief, M.

Protein structure by mechanical triangulation

Proc Natl Acad Sci U S A

Schlierf, M.;Rief, M.

Single-molecule unfolding force distributions reveal a funnel-shaped energy landscape

Biophys J

Hugel, T.;Rief, M.;Seitz, M.;Gaub, H. E.;Netz, R. R.

Highly stretched single polymers: Atomic-force-microscope experiments versus ab-initio theory

Physical Review Letters

[removed]://000226779000074

Kufer, S. K.;Dietz, H.;Albrecht, C.;Blank, K.;Kardinal, A.;Rief, M.;Gaub, H. E.

Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy

European Biophysics Journal with Biophysics Letters

[removed]://000233392200006

Clemen, A. E. M.;Vilfan, M.;Jaud, J.;Zhang, J. S.;Barmann, M.;Rief, M.

Force-dependent stepping kinetics of myosin-V

Biophysical Journal

[removed]://000229503000064

Junker, J. P.;Hell, K.;Schlierf, M.;Neupert, W.;Rief, M.

Influence of substrate binding on the mechanical stability of mouse dihydrofolate reductase

Biophysical Journal

[removed]://000232933300006

Schwaiger, I.;Schleicher, M.;Noegel, A. A.;Rief, M.

The folding pathway of a fast-folding immunoglobulin domain revealed by single-molecule mechanical experiments

Embo Reports

[removed]://000226183500011

Schlierf, M.;Rief, M.

Temperature Softening of a Protein in Single-molecule Experiments

J Mol Biol

Dietz, H.;Rief, M.

Exploring the energy landscape of GFP by single-molecule mechanical experiments

Proc Natl Acad Sci U S A

[removed]://000225226200021

Schwaiger, I.;Kardinal, A.;Schleicher, M.;Noegel, A. A.;Rief, M.

A mechanical unfolding intermediate in an actin-crosslinking protein

Nature Structural & Molecular Biology

[removed]://000220143900017

Krautbauer, R.;Rief, M.;Gaub, H. E.

Unzipping DNA oligomers

Nano Letters

[removed]://000182201800013

Zhuang, X. W.;Rief, M.

Single-molecule folding

Current Opinion in Structural Biology

[removed]://000181133300013

Rief, M.;Grubmuller, H.

Force spectroscopy of single biomolecules

Chemphyschem

[removed]://000174557400004

Schwaiger, I.;Sattler, C.;Hostetter, D. R.;Rief, M.

The myosin coiled-coil is a truly elastic protein structure

Nature Materials

[removed]://000181498800020

Rief, M.;Rock, R. S.;Mehta, A. D.;Mooseker, M. S.;Cheney, R. E.;Spudich, J. A.

Myosin-V stepping kinetics: A molecular model for processivity

Proc Natl Acad Sci U S A

[removed]://000088840500028